Human Anti-Human EPCAM Recombinant Antibody (3622W94)

Name: Human Anti-Human EPCAM Recombinant Antibody (3622W94)
SKU: TAB-235CL
Rating: 5 (1 reviews)

CAT#: TAB-235CL

Recombinant Humanized monoclonal antibody expressed in CHO binding to Human EPCAM. 3622W94 is a humanized murine monoclonal antibody (MoAb) against the 17-1A antigen, with potential adjuvant therapeutic properties in colorectal cancer.

Published Data Tested Data Gene Expression

Cyt

Figure 1 ADCC activity of five clinically tested anti-EpCAM monoclonal antibodies.

ADCC was determined by dose response analysis as shown for 2 out of 5 human PBMC donors tested.

Münz, M., Murr, A., Kvesic, M., Rau, D., Mangold, S., Pflanz, S., ... & Rüttinger, D. (2010). Side-by-side analysis of five clinically tested anti-EpCAM monoclonal antibodies. Cancer cell international, 10(1), 44.

Figure 2 Mapping of the binding domain of adecatumumab to exon 5 of human EpCAM.

FACS histograms showing the binding of adecatumumab to 293 cells expressing the EpCAM constructs.

❮

❯

ELISA

Figure 1 Anti-Human EPCAM Antibody (TAB-235CL) in ELISA

ELISA analysis of TAB-235CL was performed with Human EpCAM Protein, His Tag.
The secondary antibody: HRP-Anti-Human IgG (H+L)

Figure 2 Anti-Human EPCAM Antibody (TAB-235CL) in WB

Western blot analysis of TAB-235CL was performed with Human EpCAM Protein, His Tag.
TAB-235CL incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing antigen (0.6μg)

Figure 3 Anti-Human EPCAM Antibody (TAB-235CL) in DB

Dot blot analysis of TAB-235CL was performed with Human EpCAM Protein, His Tag.
TAB-235CL incubation concentration: 2ng/μL.
NC: Negative control PC: Positive control

ELISA

Figure 4 Anti-Human EPCAM Recombinant Antibody (TAB-235CL) in ELISA

ELISA analysis of TAB-235CL was performed by coating with human EPCAM protein (His tag).

❮

❯

Subcellular Location Figure 1 IF staining of human cell line MCF7

Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67463/1592_H11_2_selected.jpg

Normal Tissue Figure 2 Thyroid gland

Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_B_2_5.jpg

Normal Tissue Figure 3 Colon

Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_9_3.jpg

Normal Tissue Figure 4 Kidney

Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_8_5.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (A-431, CACO-2, CAPAN-2, OE19, RPTEC TERT1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119888-EPCAM

❮

❯

Specifications

Derivation
Humanized

Type
Antibody

Species Reactivity
Human

Applications
Cyt, FC

Related Disease
Cancer

Product Property

Purity
>95% by HPLC

Storage
Short term: store at 4°C (over 6 months), long term: -20°C or -80°C.

Applications

Application Notes
This antibody has been reported for use in Cytotoxicity and Flow Cytometry.

Target

Alternative Names
EPCAM; epithelial cell adhesion molecule; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated an

Gene ID
4072

UniProt ID
P16422

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

Inquiry

Navs

Customer Review

There are currently no Customer reviews or questions for TAB-235CL. Click the button above to contact us or submit your feedback about this product.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-235CL, RRID: AB_3111892)

Copy citation

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-235CL.pdf

MSDS

MSDS - TAB-235CL.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

See other products for "EPCAM"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
TAB-112-F(E)	Anti-Human EpCAM Recombinant Antibody Fab Fragment, 17-1A (Citatuzumab Bogatox)	Neut, ELISA, IF, IP, FuncS, FC, ICC	Fab - G1 - kappa
TAB-362MZ-S(P)	Anti-Human EPCAM Recombinant Antibody scFv Fragment (3622W94)	Confocal, ELISA	Humanized antibody
TAB-374MZ-S(P)	Anti-Human EPCAM Recombinant Antibody scFv Fragment (h12H8B)	FC, ELISA	Humanized antibody
TAB-375MZ-S(P)	Anti-Human EPCAM Recombinant Antibody scFv Fragment (h12H8C)	FC, ELISA	Humanized antibody
TAB-376MZ-S(P)	Anti-Human EPCAM Recombinant Antibody scFv Fragment (h2D11B)	FC, ELISA	Humanized antibody

CAT	Product Name	Application
AGTO-L013E	anti-EPCAM immunotoxin MOC31 (IgG)-PE	Cytotoxicity assay, Functional assay
AGTO-L013D	anti-EPCAM immunotoxin MOC31 (IgG)-DT	Cytotoxicity assay, Functional assay
AGTO-L013G	anti-EPCAM immunotoxin MOC31 (IgG)-Gel	Cytotoxicity assay, Functional assay
AGTO-L013R	anti-EPCAM immunotoxin MOC31 (IgG)-RTA	Cytotoxicity assay, Functional assay
AGTO-L013O	anti-EPCAM immunotoxin MOC31 (IgG)-Bouganin	Cytotoxicity assay, Functional assay

CAT	Product Name	Application	Type
TAB-356MZ	Anti-Human EPCAM Recombinant Antibody (3-171)	Cyt, ELISA, FC, WB	Human antibody
TAB-357MZ	Anti-Human EPCAM Recombinant Antibody (7-F17)	ELISA	Human antibody
TAB-358MZ	Anti-Human EPCAM Recombinant Antibody (12-C15)	ELISA	Human antibody
TAB-359MZ	Anti-Human EPCAM Recombinant Antibody (16-G5)	ELISA	Human antibody
TAB-360MZ	Anti-Human EPCAM Recombinant Antibody (17-C20)	ELISA	Human antibody

CAT	Product Name	Application	Type
TAB-363MZ	Mouse Anti-EPCAM Recombinant Antibody (TAB-363MZ)	ELISA, FuncS	Mouse IgG1, κ
TAB-364MZ	Mouse Anti-EPCAM Recombinant Antibody (TAB-364MZ)	FC	Mouse IgG1
TAB-365MZ	Mouse Anti-EPCAM Recombinant Antibody (TAB-365MZ)	FC	Mouse IgG1
TAB-366MZ	Mouse Anti-EPCAM Recombinant Antibody (TAB-366MZ)	ELISA, FC	Mouse IgG
TAB-367MZ	Mouse Anti-EPCAM Recombinant Antibody (TAB-367MZ)	FC, FuncS, Inhib, WB	Mouse IgG

CAT	Product Name	Application	Type
MOR-1163	Hi-Affi™ Rabbit Anti-EPCAM Recombinant Antibody (clone DS1163AB)	IHC-P, WB, ICC, ICC, IF	Rabbit IgG

CAT	Product Name	Application	Type
FAMAB-0095-YC-S(P)	Mouse Anti-EPCAM Recombinant Antibody (clone MOC31); scFv Fragment	ELISA	Mouse scFv
HPAB-2218LY-S(P)	Human Anti-EPCAM Recombinant Antibody; scFv Fragment (HPAB-2218LY-S(P))	ELISA	Human scFv
HPAB-2219LY-S(P)	Human Anti-EPCAM Recombinant Antibody; scFv Fragment (HPAB-2219LY-S(P))	ELISA	Human scFv
HPAB-2220LY-S(P)	Human Anti-EPCAM Recombinant Antibody; scFv Fragment (HPAB-2220LY-S(P))	ELISA	Human scFv
HPAB-2221LY-S(P)	Human Anti-EPCAM Recombinant Antibody; scFv Fragment (HPAB-2221LY-S(P))	ELISA	Human scFv

CAT	Product Name	Application	Type
MRO-0537-CN	Rabbit Anti-EPCAM Recombinant Antibody (clone CBACN-213)	WB, IHC	Rabbit IgG
HPAB-2219LY	Human Anti-EPCAM Recombinant Antibody (HPAB-2219LY)	ELISA	Human IgG
HPAB-2220LY	Human Anti-EPCAM Recombinant Antibody (HPAB-2220LY)	ELISA	Human IgG
HPAB-2221LY	Human Anti-EPCAM Recombinant Antibody (HPAB-2221LY)	ELISA	Human IgG
HPAB-2222LY	Human Anti-EPCAM Recombinant Antibody (HPAB-2222LY)	ELISA	Human IgG

CAT	Product Name	Application	Type
MRO-0538-CN	Mouse Anti-EPCAM Recombinant Antibody (clone 3-E1-E2)	WB, IF, IHC, FC	Mouse IgM

CAT	Product Name	Application	Type
AFC-TAB-106	Afuco™ Anti-EPCAM ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-106)	ELISA, IP, FC, FuncS, Neut	ADCC enhanced antibody
AFC-TAB-213	Afuco™ Anti-EPCAM ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-213)	Neut, ELISA, IF, IP, FuncS	ADCC enhanced antibody
AFC-TAB-701	Afuco™ Anti-EpCAM ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-701)	ELISA, IP, FC, FuncS, IF, Neut	ADCC enhanced antibody

CAT	Product Name	Application	Type
HPAB-2216LY-F(E)	Human Anti-EPCAM Recombinant Antibody; Fab Fragment (HPAB-2216LY-F(E))	ELISA	Human Fab
HPAB-2217LY-F(E)	Human Anti-EPCAM Recombinant Antibody; Fab Fragment (HPAB-2217LY-F(E))	ELISA	Human Fab
HPAB-2218LY-F(E)	Human Anti-EPCAM Recombinant Antibody; Fab Fragment (HPAB-2218LY-F(E))	ELISA	Human Fab
HPAB-2219LY-F(E)	Human Anti-EPCAM Recombinant Antibody; Fab Fragment (HPAB-2219LY-F(E))	ELISA	Human Fab
HPAB-2220LY-F(E)	Human Anti-EPCAM Recombinant Antibody; Fab Fragment (HPAB-2220LY-F(E))	ELISA	Human Fab

CAT	Product Name	Application	Type
MHC-CN0031	APC-H-2Db/Human Ep-CAM (CLVMKAEM) MHC Tetramer	FCM
MHC-CN0032	PE-H-2Db/Human Ep-CAM (CLVMKAEM) MHC Tetramer	FCM

CAT	Product Name	Application	Type
VS-0724-YC1517	AbPlus™ Anti-EPCAM Magnetic Beads (VS-0724-YC1517)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0924-YC37	Mouse Anti-EPCAM Recombinant Antibody (VS-0924-YC37) - Cancer Stem Cell Marker	IHC, WB	Mouse IgG1
VS-0924-YC38	Rabbit Anti-EPCAM Antibody (VS-0924-YC38) - Cancer Stem Cell Marker	IHC, WB	Rabbit IgG

CAT	Product Name	Application	Type
VS-0125-FY18	Human Anti-EpCAM (clone 3-171) scFv-Fc Chimera	Cyt, ELISA, FC, WB	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0225-XY107	CytoStream™ Mouse Anti-EPCAM Recombinant Antibody (VS-0225-XY107)	FC	Mouse IgG2b, kappa

CAT	Product Name	Application
VS-0325-XY773	Anti-EPCAM Immunohistochemistry Kit	IHC
VS-0525-XY2307	Anti-Human EPCAM Immunohistochemistry Kit	IHC
VS-0525-XY2308	Anti-Mouse EPCAM Immunohistochemistry Kit	IHC
VS-0525-XY2309	Anti-Canine EPCAM Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0425-YC210	Recombinant Anti-EPCAM Vesicular Antibody, EV Displayed (VS-0425-YC210)	ELISA, FC, Neut, Cell-uptake

CAT	Product Name	Application	Type
VS-0525-YC214	Recombinant Anti-EpCAM (Exon 2 x Exon 5) Biparatopic Antibody, Tandem scFv (Edrecolomab x Adecatumumab)	ELISA, FC	Tandem scFv

Specific Inquiry

Size:

Conjugation:

Endotoxin:

Purity:

Formats:

Isotype Switching:

Fc Engineering:

Modalities:

Add to compare

Request
Custom Production

See Our Custom Production in Action

Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE

Download the full case study

Popular Products

Figure 2 Anti-Human CD200 Recombinant Antibody (TAB-210) in HPLC

Anti-Human CD200 Recombinant Antibody (TAB-210) (CAT# TAB-210)
Application: IF, IP, Neut, FuncS, ELISA, FC, WB

Figure 2 Anti-alpha 4 beta 7 Antibody (TAB-H75) in Dot Blot

Anti-Human ITGA4+ITGB7 Recombinant Antibody (TAB-H75) (CAT# TAB-H75)
Application: FC, IP, ELISA, Neut, FuncS, IF, WB

Figure 3 Anti-Human HSP90 Recombinant Antibody (TAB-368CL) in ELISA

Anti-Human HSP90 Recombinant Antibody (CAT# TAB-368CL)
Application:

Figure 1 Human IgG4 Isotype Control Antibody (MOB-0408ZL) in SDS-PAGE

Human IgG4 Isotype Control Antibody (CAT# MOB-0408ZL)
Application: ELISA, IB, FC, IHC, FuncS

Figure 1 Rabbit Anti-Hypusine Recombinant Antibody (PABL-202) in SDS-PAGE

Rabbit Anti-Hypusine Recombinant Antibody (PABL-202) (CAT# PABL-202)
Application: WB, ELISA, FuncS

Figure 2 Recombinant Mouse Anti-N. meningitidis PorA (P1.2) Antibody (PABW-092) in HPLC

Recombinant Mouse Anti-N. meningitidis PorA (P1.2) Antibody (MN16C13F4) (CAT# PABW-092)
Application: ELISA

Figure 2 Recombinant Mouse Anti-Staphylococcal enterotoxin B Antibody (PABL-706) in HPLC

Recombinant Mouse Anti-Staphylococcal enterotoxin B Antibody (PABL-706) (CAT# PABL-706)
Application: WB, IF, FuncS

Figure 2 Anti-Human LAG3 Recombinant Antibody (TAB-0367CL) in ELISA

Anti-Human LAG3 Recombinant Antibody (TSR-033) (CAT# TAB-0367CL)
Application: FC, FuncS, Block

Figure 6 Recombinant Human Anti-H3N2 HA Antibody (F045-092) (PABC-233) in DB

Recombinant Human Anti-H3N2 HA Antibody (F045-092) (CAT# PABC-233)
Application: Neut

Figure 2 Anti-CLDN4 Antibody (TAB-501LC) in HPLC

Anti-Human CLDN4 Recombinant Antibody (KM3900) (CAT# TAB-501LC)
Application: WB, IP, FC

Figure 2 Anti-Human DEspR Recombinant Antibody (TAB-190MZ) in HPLC

Anti-Human DEspR Recombinant Antibody (TAB-190MZ) (CAT# TAB-190MZ)
Application: FC, FuncS, Inhib

Figure 2 Anti-Human FAP Antibody (TAB-024WM) in ELISA

Human Anti-FAP Recombinant Antibody (TAB-024WM) (CAT# TAB-024WM)
Application: IHC, ELISA, FC, IF

Figure 4 Anti-MUC1 Recombinant Antibody (TAB-439MZ) in WB

Anti-Human MUC1 Recombinant Antibody (1B2) (CAT# TAB-439MZ)
Application: FC

Figure 3 Anti-HIV-1 clade B gp120 Recombinant Antibody (MRO-022LC) in ELISA

Anti-HIV-1 clade B gp120 Recombinant Antibody (A32) (CAT# MRO-022LC)
Application: WB

Figure 5 Mouse Anti-FAP Recombinant Antibody (HPAB-0171-CN) in ELISA

Mouse Anti-FAP Recombinant Antibody (HPAB-0171-CN) (CAT# HPAB-0171-CN)
Application: ELISA

Figure 4 Mouse Anti-PSCA Recombinant Antibody (HPAB-0287CQ) in WB

Mouse Anti-PSCA Recombinant Antibody (clone mAb 8D11) (CAT# HPAB-0287CQ)
Application: FC, IHC, Cyt, FuncS

Figure 2 Human Anti-CA19-9 Recombinant Antibody; Fab Fragment (HPAB-0038-WJ-F(E)) in HPLC

Human Anti-CA19-9 Recombinant Antibody Fab Fragment (HPAB-0038-WJ-F(E)) (CAT# HPAB-0038-WJ-F(E))
Application: ELISA

Figure 2 Mouse Anti-CRP Recombinant Antibody; Fab Fragment (HPAB-M0039-YC-F(E)) in ELISA

Mouse Anti-CRP Recombinant Antibody (clone P24); Fab Fragment (CAT# HPAB-M0039-YC-F(E))
Application: ELISA

Figure 2 Human Anti-HLA-DR4 Recombinant Antibody (HPAB-0827-FY) in HPLC

Human Anti-HLA-DR4/GAD-555-567 Recombinant Antibody (clone G1H12) (CAT# HPAB-0827-FY)
Application: FC

Figure 4 Mouse Anti-ACTA2 Antibody (ZG-1036F) in IHC

Mouse Anti-ACTA2 Recombinant Antibody (clone HHF35) (CAT# ZG-1036F)
Application: EM, ELISA, ICC, IHC-Fr, IHC-P, WB

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.